Reversed Phase HPLC: practical and theoretical approaches to empower the day to day analyst working in Industry

Fechas: 1, 08, 16 y 29 de octubre de 2018

Horarios: lunes (1, 08 y 29) y martes (16) de 17 a 20 horas

Lugar: Aula Píriz McColl. Instituto de Química (Gral. Flores 2124)

Docente invitada: Dra. Arianne Soliven, PhD en Química Analítica (Western Sydney University), con más de 10 años de experiencia en cromatografía, tanto en la Academia como en la industria y con numerosas publicaciones en revistas referadas.

Idioma: Inglés

Summary of short course proposal:

Theory-Practical (majority in classroom lessons/discussions)

Duration: 12 hrs in total (3 hrs/wk for 4 weeks attendance)

Audience: Analysts of all levels, preferable experience executing 1 method.

Objective: Analyst’s to understand their operational procedures, and develop a practical and theoretical understanding for better execution and rapid troubleshooting

Format: Interactive, lectures and group discussions. Students/professionals are encouraged to implement their applications and experiences to add value to their understanding

o Classroom lectures 70%

o In class group discussions/assignments: 20%

o Practical 10% (1 hour in the lab visually inspecting instruments)

Topics:

1. LC system: responsibilities of each module

2. Column selection

3. Understanding the method and choice in consumables

4. A practical and theoretical approach for executing protocols successfully and fast troubleshooting

Topics and class structure outline:

Week 1. The LC system: responsibilities of each module a. 1st hour in lab (no operational use/concumables used just visually looking at the systems and their parts)

b. Open all modules and compartments and reveal and explain each part

c. General overview and explanation of the practical responsibility of each module: pump, mixer, injector, column, detector

d. How does each module effect the system and the chromatogram

e. In classroom: looking at the design of different vendor systems

f. 1st week in class discussion/assignment: schematic diagram of a system and how they influence the chromatogram

Week 2. Column selection

a. Participants asked to share the details of 3 columns they often use

b. The column is the heart of separation – general overview of different formats and their advantages and disadvantages

c. Opening a catalogue together – large column selection choice –how to understand the parameters and how they relate to the separation e.g. carbon load, surface area, porosity etc.

d. Batch to batch differences

e. Understanding the how column selection effects separation

f. 2nd week assignment in class group discussion/assignment: understanding the

common column choices and alternatives

Week 3. Understanding the method and choice in consumables

a. Participants asked to share if they perform any basic quick checks/tests to verify instrument and column performance

b. Importance of tubing and module connections

c. Vial choice – how it can effect quantitation

d. Mobile phase and additives

e. Detector choice and settings

f. Injector program

g. Cleaning methods

3rd week assignment in class group discussion/assignment: creating day-to-day simple verification/performances checks?

Week 4. A practical and theoretical approach for executing protocols successfully and fast troubleshooting.

a. Participants asked to briefly share approaches to execute protocols and errors

b. Introduce theoretical equations (not memorise) which related to understanding the instrument, column and method

c. A systematic approach to troubleshooting

d. Understanding everything and all artifacts in a chromatogram

4th week assignment in class group discussion/assignment: Dissect established methods their steps from a practical and theoretical approach and and possible errors

Costo: $ 2.000

Inscripciones completando la siguiente ficha de inscripción

La matrícula se deben abonar en la Sección Tesorería de Facultad de Química (Isidoro de María 1614 planta baja, de lunes a viernes de 10 a 13 hrs), o realizando depósito en el BROU (cuenta corriente nº 00155946300004), el talón se debe enviar por correo electrónico a Esta dirección de correo electrónico está siendo protegida contra los robots de spam. Necesita tener JavaScript habilitado para poder verlo.